Both Radiative and chemiluminescent detection chemistries are offered. Describes which blotting technique is appropriate based on the type of macromolecule. The size of the transcript will be estimated and the presence of partial transcripts will be reported. Although this technique is quite labor-intensive, it's particularly useful for detecting large deletions in tumor genomes. Transcript Analysis by Northern Blot service is offered to detect the presence of an expressed transcript containing a sequence of interest. Southern blotting targets DNA, northern blotting target is RNA and western blot targets are protein. Southern blot analysis can be used to investigate whether a gene is amplified, deleted, or structurally rearranged in cancer cells as compared to normal cells. We offer Southern blot analysis to support gene discovery and confirmation, gene mapping, evolution and development studies, diagnostics, and forensics tests. Northern blots confirm the gene expression pattern of a cell bank.Īvance Biosciences provides all necessary expertise for Southern or Northern blot projects from nucleotide extraction to probe design. Southern blots also confirm target identity as matching the hybridization probe, size as compared to a molecular weight marker, and abundance. Southern blotting was invented before PCR, but PCR has replaced blotting in many applications because of its simplicity, speed, and convenience. The FDA requires Southern blots to confirm that the structure of the genomic DNA of a cell bank is stable and unchanged and that no clonal rearrangement has occurred. Products derived from transgenic organisms, for use as human or animal drugs or for biologics are regulated by the FDA for safety and efficacy.
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